Sequencing Service - Faculty of Biology

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News
02.01.24: Current delivery times in the Biocenter
As always, we remain open and fully operational. The deadline for delivery of sequencing samples stays at 10:00 AM for same-day service.
If you need your results more quickly please contact Ute Bergmann under 089/2180.74715 or seqservice@bio.lmu.de for individual handling of your samples. We are happy to help whenever possible to provide you with optimal turnaround time.

19.08.22: Problems with colored PCR tubes
Recently, we discovered severe problems with colored PCR tubes. Apparently, there's a new product which leaches fluorescent molecules into the sequencing reaction during sequencing, making detection of the sequencing products impossible.
Although this seems to not happen with all colors, we'd advise you to better use only clear PCR tubes.


Welcome
Welcome to the Sequencing Service of the Genomics Service Unit


We are offering the following services to academic research groups:

Cycle, Clean & Run: You provide us with a template/primer mix, we do the sequencing reaction, clean-up of the reaction, and the sequencing run.

Clean & Run: You do the sequencing reaction yourself, we perform clean-up and the sequencing run.

Run: You do the sequencing reaction and clean-up yourself, we only perform the sequencing run (only available for full 96 well plates).

Fragment Analysis Run: You do the PCR reactions, dilutions and mixing with length standard, we do the run (only available for full 96 well plates).

For sequencing services you have the choice between BigDye v1.1 and BigDye v3.1 sequencing chemistry. For fragment analysis we have currently established three dye sets:
Dye Set G5 with 6-FAM, PET, NED, and VIC (size standard: LIZ)
Dye Set F with 5-FAM, JOE, and NED (size standard: ROX)
Dye Set E5 with dR6G, dR110, dTAMRA, and dROX for use in the Applied Biosystems SNaPshot Multiplexing System (size standard: LIZ).
Your samples are analyzed on an ABI 3730 48 capillary sequencer with 50 cm capillary length providing excellent fragment separation and sensitivity. Sequence files have usually at least 750 nucleotides of reliable data, in most cases much more.

We process samples daily from Monday to Friday (except for holidays). Samples that are deposited in the refrigerator in room G03.031 (Biocenter in Martinsried) by 10:00 AM will be processed within the same day, and data files can usually be downloaded in the evening of the same day. You can also send samples by mail, of course. Our address is: Sequencing Service, LMU Biozentrum, Großhaderner Str. 2-4, 82152 Martinsried. Please make sure that your samples are nicely padded for transport.

On the left hand side of this page you can find a tutorial, which will explain how to navigate this site, protocols for preparation of samples, and other useful files.

We very much welcome your questions, comments, ideas, and criticism under seqservice@bio.lmu.de.


Your Genomics Service Unit:
Gisela Brinkmann (089-2180.74715)
Ute Bergmann (089-2180.74715)
Andreas Brachmann (089-2180.74703)