Sequencing Service - Faculty of Biology

Below you find the requirements for sample submission, protocols for the setup of sequencing reactions, and links to free chromatogram viewers

• Requirements for sample submission (for Cycle, Clean & Run)
  Cycle, Clean & Run
  
  
• A short but tested sequencing protocol for the ABI 3730, together with requirements for sample submission (for Clean & Run)
  Clean & Run
  
  
• Requirements for sample submission (for Run)
  Run
  
  
  
• Instructions for sequencing of difficult regions, eg. GC-rich templates or homopolymer stretches (from Applied Biosystems)
  Sequencing Difficult Templates
  
  
• Frequently Asked Questions about BigDye v1.1 versus v3.1
  BigDyes FAQ


• A detailed Sequencing Chemistry Guide by Applied Biosystems
  Sequencing Chemistry Guide


• The detailed original protocol for BigDye v3.1 by Applied Biosystems
  BigDye v3.1 Protocol


• The detailed original protocol for BigDye v1.1 by Applied Biosystems
  BigDye v1.1 Protocol



• Free Chromatogram Viewers
  ApE - A plasmid Editor (for Windows and MacOSX, recommended)
  4Peaks (for MacOS X)
  Chromas Lite (for Windows)
  FinchTV (for Windows and MacOSX)
  
  
• Free Fragment Analysis Program
  Peak Scanner Software v1.0 (for Windows)
  
  
• Free Sequence Assembly and Alignment Program Package
  Staden (for MacOS X, Unix and Windows)
  
  
• Installation Files for CLC Main Workbench (License required!)
  Version 23.0.5 for PC (64 bit)
  Version 23.0.5 for Mac
  
  
• Data Files for CLC Bioinformatics Training Courses
  Tutorials for CLC Bioinformatics Software (ZIP Folder)
  Illumina/Solexa paired end genome data from E. coli commensal strain K-12 (ZIP Folder)
  CLC formatted mapping data (ZIP Folder)
  Subset of the full data set (ZIP Folder)
  454 data set (ZIP Folder)